ISO 9308-1:2014
2014-09
Abstract
ISO 9308-1:2014 specifies a method for the enumeration of Escherichia coli (E. coli) and coliform bacteria. The method is based on membrane filtration, subsequent culture on a chromogenic coliform agar medium, and calculation of the number of target organisms in the sample. Due to the low selectivity of the differential agar medium, background growth can interfere with the reliable enumeration of E. coli and coliform bacteria, for example, in surface waters or shallow well waters. This method is not suitable for these types of water.
ISO 9308-1:2014 is especially suitable for waters with low bacterial numbers that will cause less than 100 total colonies on chromogenic coliform agar (CCA). These may be drinking water, disinfected pool water, or finished water from drinking water treatment plants.
Some strains of E. coli which are β-D-glucuronidase negative, such as Escherichia coli O157, will not be detected as E. coli. As they are β-D-galactosidase positive, they will appear as coliform bacteria on this chromogenic agar.
General information
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Status: PublishedPublication date: 2014-09Stage: International Standard under systematic review [90.20]
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Edition: 3Number of pages: 10
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Technical Committee :ISO/TC 147/SC 4ICS :07.100.20
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Amendments
Amendments are issued when it is found that new material may need to be added to an existing standardization document. They may also include editorial or technical corrections to be applied to the existing document.
Amendment 1
Edition 2016
Life cycle
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Previously
WithdrawnISO 9308-1:2000
WithdrawnISO 9308-1:2000/Cor 1:2007
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Now
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Preliminary
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10
Proposal
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20
Preparatory
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30
Committee
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Enquiry
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50
Approval
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60
Publication
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90
Review
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95
Withdrawal
Amendments
Provide additional content; available for purchase; not included in the text of the existing standard.PublishedISO 9308-1:2014/Amd 1:2016
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